STAT3 Regulates Proliferation and Survival of CD8+ T Cells: Enhances Effector Responses to HSV-1 Infection, and Inhibits IL-10+ Regulatory CD8+ T Cells in Autoimmune Uveitis
Figure 3
Enhanced proliferation and sensitivity to AICD of STAT3-deficient CD8+ T cells correlated with alterations in the expression of proapoptotic, antiapoptosis, and cell-cycle regulatory genes. Naïve CD8+ T cells were cultured for 4 days under Tc0 and Tc1 polarization condition. RNA was isolated and analyzed by RT-PCR (a, c, d) or qRT-PCR (b, e). (f) Naïve CD8+ T cells were cultured for 4 days under non-polarizing condition, and expression of OX-40 was detected by FACS. Numbers in quadrants represent percentage of CD8+ T cells expressing OX-40. Data represent at least 3 independent experiments.
