STAT3 Regulates Proliferation and Survival of CD8+ T Cells: Enhances Effector Responses to HSV-1 Infection, and Inhibits IL-10+ Regulatory CD8+ T Cells in Autoimmune Uveitis
Figure 5
STAT3KO mice induce expansion of IL-10-producing regulatory CD8+ T cells during EAU. (a–d) WT or STAT3KO mice were immunized with IRBP in CFA. (a) Fundus images taken on day-21 after immunization. (b) and 21 days after immunization. Freshly isolated LN cells (21 days after immunization) were analyzed by the intracellular cytokine staining (b, c). CD3+ T cells were gated, and numbers in quadrants indicate percentage of CD4+ or CD8+ T cells expressing IL-17A or IL-10. (d, e) CD8+ T cells were isolated from the LN by magnetic sorting, and RNA was analyzed for IL-10 expression by RT-qPCR (d) or RT-qPCR (e). Data represent at least 3 independent experiments.
