Human Endothelial-Like Differentiated Precursor Cells Maintain Their Endothelial Characteristics When Cocultured with Mesenchymal Stem Cell and Seeded onto Human Cancellous Bone
EPC on Tutoplast, decline of DiLDL uptake over the observation period independently of the presence of osteogenic stimulation. Representative images of day 2, day 6, and day 10 under control conditions (a) and osteogenic conditions (b), and data evaluations (c) were shown. Parallel preparations were used. The DiLDL staining was performed on the day of measurement. Thus, a reduced DiLDL fluorescence is not due to a fading effect but due to a reduced uptake of DiLDL. The exposure time was 0.25 s. Scale bar indicates 100 μm.
(a) EPC, control
(b) EPC, osteogenic stimulation
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