Figure 3: Levels of phosphoextracellular signal-regulated kinase (ERK1/2 p44/p42) in mouse 3T3-L1 adipocytes. Cells were pretreated for 24 h with alliin 0.1 mM and subsequently exposed to 100 ng/mL of lipopolysaccharides (LPS) for 1 h afterward. (a) Representative Western blot with phospho-ERK1/2 and ERK1/2 antibodies; (b) protein levels of phospho-ERK1/2 and ERK1/2 in total cell extracts. CT control; AU arbitrary units. Data are expressed as mean ± standard deviations (SD) of three independent experiments * ; ** .