Heat Shock Proteins 60 and 70 Specific Proinflammatory and Cytotoxic Response of CD4+CD28null Cells in Chronic Kidney Disease
Figure 1
Representative image of ELISPOT analysis in patients with CKD (a) and healthy subjects (b): [1–4] CD4+CD28null cells and [5–8] CD4+CD28+ cells stimulated with and without PHA (10 g/mL), HSP60 (5 ng/mL), and HSP70 (5 ng/mL), respectively. (A) Interferon- spots, (B) perforin spots, and (C) granzyme B spots. CD28null cells responded strongly to HSP60 and HSP70 as compared to CD28+ cells as shown by the spot development for IFN-, perforin, and granzyme B in CKD subjects whereas there was no response as shown by lack of spot development for IFN-, perforin, and granzyme B in healthy controls. However, the cells responded robustly to PHA, a positive control as shown by the interferon-, perforin, and granzyme B spots.