Leptomeningeal Cells Transduce Peripheral Macrophages Inflammatory Signal to Microglia in Reponse to Porphyromonas gingivalis LPS

Figure 3

Secretion of proinflammatory mediators by microglia after treatment with the conditioned medium from P.g. LPS-treated leptomeningeal cells and P.g. LPS. The mean mRNA levels of TNF-α and IL-1β of MG6 microglia at 24 h incubated with P.g. LPS (LPS) and the conditioned medium of leptomeningeal cells (LCM) after treatment with P.g. LPS (a). Data are presented by mean ± SEM (𝑛=3), *𝑃<0.05, ***𝑃<0.001 versus nontreated cells (none). ^†††𝑃<0.001 versus P.g. LPS alone. The P.g. LPS-induced TNF-α secretion in MG6 microglia at 48 h with the neutralizing antibodies against TLR2 (10 μg/mL), TLR4 (10 μg/mL), or Bay 11-7082 (Bay, 20 μM) (b). Data are presented by mean ± SEM (𝑛=4, each), ***𝑃<0.001 versus nontreated cells (none), and ^†††𝑃<0.001 versus P.g. LPS alone.

(a)

(b)