Research Article

Hyperoxia Exacerbates Postnatal Inflammation-Induced Lung Injury in Neonatal BRP-39 Null Mutant Mice Promoting the M1 Macrophage Phenotype

Figure 1

Hyperoxia promotes the M1 phenotype in macrophages. Inducible nitric oxide synthase (iNOS) and interleukin-6 (IL-6) mRNA expression were measured by real-time PCR in the RAW264.7 macrophages after stimulation with lipopolysaccharide (LPS; 100 ng/mL) for 16 h in room air (RA) or 95% hyperoxia (HYP) ((a) and (b)). Representative Western blot showing 24 h LPS mediated protein induction of iNOS in RA and HYP groups (c). IL-1β and IL-10 were measured by ELISA in the supernatants of macrophages after stimulation for 24 h with LPS ((d)-(e)). Results expressed as the mean ± SEM of data obtained from three independent experiments. C: control (RA). , , and .