A Subanesthetic Dose of Isoflurane during Postconditioning Ameliorates Zymosan-Induced Neutrophil Inflammation Lung Injury and Mortality in Mice

Figure 8

ISO at 0.7% inhibited ZY-induced NF-κB activation, iNOS activity, and NO production in neutrophils. (a) NF-κB DNA-binding activity. (b) iNOS mRNA expression as assessed by qPCR. (c) iNOS enzyme activity. (d) Assay of nitrite levels as an indicator of NO generation. The neutrophils were treated as shown in Figure 1(b). Data represent means ± SD for triplicates of each group.^#𝑃<0.05versus ZY + vehicle (ZY).^§𝑃<0.05versus ZY + ISO. NAI, NF-κB activation inhibitor. DMSO is provided as a solvent control for NAI.

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