Figure 3: Effect of resistin on mRNA levels of lipid metabolism enzymes and proinflammatory cytokines in vitro. Mean + SEM mRNA levels of ACCα, CPT-1, FAS, LPL, MCD, SCD-1, SREBP, IL-6, and TNF-alpha. After 3 days of culture, dispersed rat pituitary cells were incubated in medium alone (C, Control) or in the presence of resistin (10−14–10−6 M) for 4 h. After culture, cells were harvested, and receptor mRNA levels were determined by real-time RT-PCR. Enzymes band intensities were determined and adjusted by the signal intensity for HPRT. The averaged results were then calculated and expressed as a percentage of vehicle-treated control levels (100%). Data are the mean (±SEM) of three separate experiments. At least three replicate wells were evaluated per treatment in each experiment. * versus corresponding control.