Mediators of Inflammation / 2013 / Article / Tab 1 / Research Article
IL-33/ST2 Pathway and Classical Cytokines in End-Stage Heart Failure Patients Submitted to Left Ventricular Assist Device Support: A Paradoxic Role for Inflammatory Mediators? Table 1 Analytical details of gene primers for real-time PCR analysis.
Sequence GenBank, accession number Length (bp) Temp (°C) Efficiency (%)
ST2 Forward CTCCAAGTTCATCCCCTCT NM_000877.2 110 60 103.1 0.991 Reverse GATCCAAAACCCCATTCTGTT IL-33 Forward GGAGTGCTTTGCCTTTGGTA NM_033439 140 60 100.2 0.991 Reverse TCATTTGAGGGGTGTTGAGA IL-8 Forward CCAAGCTGGCCGTGGCTTCTC NM_000584 185 64.5 100.6 0.997 Reverse TGTGTTGGCGCAGTGTGGTCC IL-6 Forward AGCGCCTTCGGTCCAGTTGC NM_000600 121 64.5 100 0.999 Reverse GTGGCTGTCTGTGTGGGGCG TNF-
Forward TCCTCAGCCTCTTCTCCTTC NM_000594.2 279 58 119.8 0.997 Reverse CCAGCTGGTTATCTCTCA RPL13a Forward CGCCCTACGACAAGAAAAAG NM_012423 206 60 104.6 0.999 Reverse CCGTAGCCTCATGAGCTGTT PPIA Forward CTTGGGCCGCGTCTCCTTCG NM_021130 285 60 103.4 0.998 Reverse TTGGGAACCGTTTGTGTTTGGGGC YWHAZ Forward ATGCAACCAACACATCCTATC NM_00113572 178 60 95.3 0.997 Reverse GCATTATTAGCGTGCTGTCTT
