Figure 3: PA induces nuclear translocation of NF-κB p65 subunit in HaCaT cells measured by western blotting. Keratinocytes were untreated or treated with PA (75, 100, 125, and 150 μmol/L) for 24 hours under serum-free conditions. The cells were harvested, and nuclear extracts were analyzed with NF-κB p65 by western blot immediately after the treatment (a) and 24 hours after the PA depletion (c). The densitometry values are means ± SD of three independent experiments ((b) and (d)). Asterisks (*) indicate significant differences of , respectively, between the PA-treated groups and nontreated group.