Research Article

IL-21 Modulates Release of Proinflammatory Cytokines in LPS-Stimulated Macrophages through Distinct Signaling Pathways

Figure 1

Effects of IL-21 on LPS-induced IL-21R and γc mRNA expression in mouse peritoneal macrophages. Mouse peritoneal macrophages were incubated with IL-21 (100 ng/mL) in the presence or absence of LPS (100 ng/mL) for 6 h. Macrophages were stained with IgG2b and CD11b antibodies (a). Total RNA was isolated and analyzed by real time RT-PCR for IL-21R (c) and γc (d). IL-21R and γc expression was normalized to endogenous control GAPDH. Results are expressed as mean ± SD for each group from three independent experiments.
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