IL-21 Modulates Release of Proinflammatory Cytokines in LPS-Stimulated Macrophages through Distinct Signaling Pathways

Figure 7

Effects of IL-21 on LPS-induced expression of cell surface markers in mouse peritoneal macrophages. Macrophages (1×10⁶) were incubated with IL-21 (100 ng/mL) in the presence or absence of LPS (100 ng/mL) for 24 h. (a) and (b) Gating strategy used to identify M1 macrophages present in peritoneal macrophages based on surface molecules CD11b and CD86. (c)–(e) Total proteins from macrophages were analyzed by Western blot with specific antibodies. Detection of Actin was estimated as protein-loading control for each lane. Values are presented as mean ± SD of three independent experiments. ^∗𝑃<0.05, ^∗∗𝑃<0.01 compared with the LPS group.

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