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Mediators of Inflammation
Volume 2013 (2013), Article ID 548073, 12 pages
Research Article

IL-21 Modulates Release of Proinflammatory Cytokines in LPS-Stimulated Macrophages through Distinct Signaling Pathways

1College of Veterinary Medicine, Jilin University, Changchun 130062, China
2College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China

Received 21 September 2013; Revised 19 November 2013; Accepted 19 November 2013

Academic Editor: Yves Denizot

Copyright © 2013 Su-nan Li et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplement 1: U0126 and BAY11-7082 inhibits IL-21-induced up-regulation of cytokine production in mouse peritoneal macrophages. Macrophages (1 × 106) were pretreated with U0126 (ERK-1/2 inhibitor) and/or BAY11-7082 (NF-κB inhibitor) for 60 min prior to stimulation with IL-21 (100 ng/ml) and/or LPS (100 ng/ml) for 12 h and 24 h. Proteins expression of IL-6 and TNF-α were evaluated by ELISA, respectively. Values are presented as mean ± SD of three independent experiments. #P < 0.001 significantly different from the NT group; *P < 0.05, * *P < 0.01 compared with the LPS group.

Supplement 2: Effects of various concentration IL-21 on LPS-induced IL-6 secretion in mouse peritoneal macrophage culture supernatants Macrophages were incubated with IL-21 (20–500 ng/ml) in the presence or absence of LPS (100 ng/ml) for 12 h and 24 h. The concentration of IL-6 was measured by ELISA. Values are presented as mean ± SD of three independent experiments.

  1. Supplementary Material