Dysregulated Circulating Dendritic Cell Function in Ulcerative Colitis Is Partially Restored by Probiotic Strain Lactobacillus casei Shirota
Figure 3
LcS restored “normal” DC stimulatory capacity in UC. (a) Identification of blood-enriched DC according to flow cytometry forward and side scatter plot and summary graphs representing mean ± SEM proportions of DC expressing CD40 and CD86 following conditioning with medium only, live LcS or dead LcS (). Separate experiments were carried out conditioning DC with LPS (). (b) Dose response T cell proliferation following MLR with control- and UC-DC (). After paired two-way ANOVA analysis (corrected with Bonferroni correction for multiple comparisons), the DC concentration was statistically significant in all cases (). Control- and UC-DC stimulatory capacity was increased following LcS conditioning at LcS concentrations of 1 × 105 (control: at 2%, UC: at 1%, at 2%, at 3% DC), 1 × 106 (control: at 2% and 3%, UC: at 2%, at 3% DC), and 1 × 107 (control: at 1%, 2%, and 3%, UC: at 2% and 3%) CFU/mL. (c) There were no significant differences between the stimulatory capacity of control and LcS-conditioned UC-DC at any LcS concentration.
