Dysregulated Circulating Dendritic Cell Function in Ulcerative Colitis Is Partially Restored by Probiotic Strain Lactobacillus casei Shirota
Figure 4
LcS conditioning of DC enhanced expression of CLA on stimulated T cells in healthy controls but not UC. (a) Histograms of CLA expression by dividing T cells, following stimulation by 3% DC from healthy control (no LcS) and after conditioning with 1 × 105/1 × 106/1 × 107 CFU/mL LcS. Example is from one experiment but representative of 3 independent experiments with similar results. (b) Summary graphs of all experiments, representing proportions of CLA+ T cells stimulated by 3% control and UC-DC conditioned with increasing doses of LcS (). One-way ANOVA was applied; value <0.05 was considered statistically significant (*, **). (c) CLA/β7 dot plots of dividing T cells, following stimulation by 3% control DC, control DC + 1 × 107 LcS, or UC-DC (no LcS). Example is from one experiment but representative of 3 independent experiments with similar results. Numbers over each dot plot represent proportion of dividing T cells expressing β7 only, β7 and CLA, CLA only, or either β7 or CLA. (d) Summary graph of all experiments () representing the proportion of dividing T cells (stimulated by 3% DC in all cases) expressing CLA only (i.e., β7 negative), out of total CLA+ dividing T cells. Paired -test was applied; value <0.05 was considered statistically significant (*, **).
