Alcohol stimulation of tracheal epithelial CBF is enhanced in mice expressing an ADMA-degrading enzyme. Tracheal rings from mice overexpressing the ADMA-degrading enzyme dimethylaminohydrolase (DDAH) were treated with 100 mM alcohol (EtOH) and their CBF compared to wild type (WT) mice. While the magnitude of maximal alcohol-stimulated CBF increases did not differ between mice, the time to maximal CBF was significantly decreased in the DDAH mice versus WT mice treated with alcohol (). No significant differences in CBF were observed in control, media-incubated tracheal rings. Bars represent SEM of triplicate separate experiments ().
