Alcohol-stimulated increases in lung slice PKA activity are enhanced in DDAH mice. Precision-cut lung slices from both wild type (WT) and dimethylaminohydrolase (DDAH) expressing mice were treated with alcohol, and their maximal PKA activity was recorded. Alcohol (EtOH; 100 mM) significantly stimulated PKA in slices from both mice at 1 hr. Alcohol-stimulated PKA was earlier in DDAH (15–30 min) versus WT lung slices (1 hr). Positive control (+) represents a 30 min treatment with 10 μM 8Br-cAMP. Bars represent SEM of triplicate separate experiments ().
