NAG-1/GDF15 Transgenic Mouse Has Less White Adipose Tissue and a Reduced Inflammatory Response
Figure 4
NAG-1 does not affect inflammatory cytokine production from Kupffer cells. (a) Kuppfer cells isolated from the livers of WT mice were plated in triplicate onto 6-well plates and cultured for 24 hrs in RPMI. Media were collected at the beginning of an experiment and cells were then refed and incubated with media containing recombinant human GDF-15 (50 ng/mL) or vehicle for 2 hours and then LPS (10 ng/mL) or vehicle for 6 hours, prior to media collection. TNF-α and IL-6 levels in the media were measured in duplicate using cytokine-specific ELISAs. The mean concentrations for each treatment are plotted. Error bars represent standard error. (b) Kupffer cells isolated from the livers of WT or mice were plated in triplicate onto 6-well plates and cultured for 24 hrs in RPMI. Media were collected at the beginning of an experiment and cells were re-fed and incubated with media containing LPS (10 ng/mL) or vehicle and incubated for 6 hours prior to collection of media. TNF-α and IL-6 levels in the media were measured in duplicate using cytokine-specific ELISAs. The mean concentrations for each treatment are plotted. Error bars represent the standard error of the mean.
