The Role of EGFR/PI3K/Akt/cyclinD1 Signaling Pathway in Acquired Middle Ear Cholesteatoma
Figure 4
EACKs were treated with 15 ng/mL of EGF for the indicated periods of time. Cells proliferation was detected by MTT assay (a). EACKs were pretreated with AG1478 or wortmannin (WTM) for 1 h and then exposed to 15 ng/mL of EGF for the indicated periods of time. Cells proliferation was detected by MTT assay (b). EACKs were incubated with or without AG1478 or wortmannin (WTM) for 1 h and then exposed to 15 ng/mL of EGF for 72 h. Cells were harvested for cell cycle analysis by flow cytometry (c). Results were summarized as mean ± SD and represent at least three independent experiments.
