Monocytes, Peripheral Blood Mononuclear Cells, and THP-1 Cells Exhibit Different Cytokine Expression Patterns following Stimulation with Lipopolysaccharide
Figure 5
Effect of mediator modulation on HUVEC activation. Whole blood was stimulated with 100 ng/mL LPS from E. coli for 4 h and diluted tenfold with M199 to obtain conditioned medium derived from blood (CMB). THP-1 cells were stimulated with 10 ng/mL LPS from E. coli for 4 h in medium M199 supplemented with 10 vol% human plasma to yield conditioned medium derived from THP-1 cells (CMT). HUVEC were stimulated with CMB and CMT, respectively. Concentrations of TNF-α, IL-6 and IL-8 prior to mediator modulation (CM) and after specific TNF-α adsorption or selective cytokine adsorption (PS-DVB) are shown. Data are given as mean of three experiments ± standard deviation. Significance was accepted at . n.s.: not significant.
