Protective Effects of Necrostatin-1 against Concanavalin A-Induced Acute Hepatic Injury in Mice

Table 1

Nucleotide sequences of primers used for PCR.

Gene	Primer	Sequence (5^′-3^′)	Melting temperature (°C)	Product size (bp)
TNF-𝛼	Forward	CAGGCGGTGCCTATGTCTC	62.2	89
	Reverse	CGATCACCCCGAAGTTCAGTAG	62.1
IFN-𝛾	Forward	GCCACGGCACAGTCATTGA	62.9	201
	Reverse	TGCTGATGGCCTGATTGTCTT	61.8
IL-2	Forward	TGAGCAGGATGGAGAATTACAGG	61.4	120
	Reverse	GTCCAAGTTCATCTTCTAGGCAC	60.6
IL-4	Forward	GGTCTCAACCCCCAGCTAGT	62.8	102
	Reverse	GCCGATGATCTCTCTCAAGTGAT	61.5
IL-6	Forward	CTGCAAGAGACTTCCATCCAG	60.1	131
	Reverse	AGTGGTATAGACAGGTCTGTTGG	60.8
IL-10	Forward	CTTACTGACTGGCATGAGGATCA	61.4	101
	Reverse	GCAGCTCTAGGAGCATGTGG	62.4
GAPDH	Forward	AGGTCGGTGTGAACGGATTTG	62.6	95
	Reverse	GGGGTCGTTGATGGCAACA	62.6