Hmgb1-IL-23-IL-17-IL-6-Stat3 Axis Promotes Tumor Growth in Murine Models of Melanoma
IL-17 is predominantly produced by γ δ T cells, and depletion of γ δ T cells inhibited tumor growth. TIL were collected from B16-F10 tumor tissues 2 weeks after B16-F10 tumor cell inoculation. IL-17-producing cells in each T-cell subset were detected by intracellular staining assay. (a) The percentage of cells producing IL-17 (). (b) Wild-type mice were inoculated with B16-F10 tumor cells and injected i.p. with normal rat-IgG or a rat anti-mouse γ δ TCR mAb or anti-mouse NKG2D mAb (100 μg/mouse) on days 0, 1, 6, 10, and 14 (). Then tumor sizes were monitored. (c) IL-17 relative mRNA expression in tumor tissues from B16-F10 tumor-bearing mice treated with rat-IgG, -γ δ TCR, or -NKG2D mAb (). (d) The percentage of cells producing IL-17 in control, anti-γ δ TCR, and anti-NKG2D treated group (). (e) Tumor growth in B16 tumor cells inoculated mice from WT MDSC, WT + anti-IL-17 MDSC, WT + Ad-IL-17 MDSC, and WT + Ad-IL-17 MDSC + anti-γ δ TCR groups (). The data show means ± SEM of tumor size and are representative of three independent experiments. .
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