Regulatory Role of GSK-3β on NF-κB, Nitric Oxide, and TNF-α in Group A Streptococcal Infection
GSK-3 inhibitor, LiCl, can reduce the mortality induced by NZ131 infection in mice and suppress the TNF-α level in sera after NZ131 infection. (a) LiCl (10 mg/kg) was intraperitoneally administered immediately and at 12, 24, and 36 h after the inoculation of NZ131 (3 × 108 CFU) into the air pouch created on the back of BALB/c mice ( per group). The Kaplan-Meier method was used to determine the difference of survival rate after NZ131 infection. P value was analyzed by log rank test (). (b) LiCl was given intraperitoneally once immediately after inoculation of NZ131 into air pouch and mice were sacrificed 24 h after infection when serum was collected for measurement of TNF-α ( in NZ131 alone group, in LiCl 50 mg/kg group, and in 100 mg/kg group).