Cannabinoid Receptor 2 Protects against Acute Experimental Sepsis in Mice

Figure 3

CB2R partially mediates the inhibitory effects of GW405833 on ConA-induced TNF-𝛼 and IL-6 production in splenocytes. (a)-(b) Splenocytes isolated from CB2R^+/+ mice were treated with vehicle or ConA (5 𝜇g/mL) in the absence or presence of GW405833 (1–10 𝜇M) for 24 h. The levels of IL-6 and TNF-𝛼 in the supernatants were detected by ELISA. Data are means ± SD (𝑛=3), *𝑃<0.05, and **𝑃<0.01 versus ConA. (c)-(d) Splenocytes isolated from CB2R^+/+ and CB2R^−/− mice were treated with ConA (5 𝜇g/mL) in the absence or presence of GW405833 (10 𝜇M) for 24 h. The levels of IL-6 and TNF-𝛼 in the supernatants were detected by ELISA. Data are means ± SD (𝑛=3), *𝑃<0.05, and **𝑃<0.01.

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