Cannabinoid Receptor 2 Protects against Acute Experimental Sepsis in Mice

Figure 4

CB2R is critical for the inhibitory role of GW405833 on LPS-triggered IL-6, TNF-𝛼, and HMGB1 production in macrophages. (a)–(c) Peritoneal macrophages isolated from CB2R^+/+ mice were treated with vehicle or LPS (1 ng/mL) in the absence or presence of GW405833 (1–10 𝜇M) for 24 h. The levels of IL-6, TNF-𝛼, and HMGB1 in the supernatants were detected by ELISA. (d)–(f) Peritoneal macrophages isolated from CB2R^−/− mice were treated as described in (a)–(c). The levels of IL-6, TNF-𝛼, and HMGB1 in the supernatants were detected by ELISA. Data are means ± SD (𝑛=3), *𝑃<0.05, and **𝑃<0.01 versus LPS.

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