Follistatin-Like 1: A Potential Mediator of Inflammation in Obesity

Figure 6

FSTL1 activates proinflammatory signaling pathways in 3T3-L1 adipocytes. Differentiated 3T3-L1 adipocytes were treated with recombinant mouse FSTL1 (100 ng/mL) for indicated time periods (0–2 h). (a) Expression and phosphorylation of IKK𝛽, NF𝜅B, and JNK were assessed by Western blot analysis. (b) and (c) Intensity of bands was quantified by densitometry and expressed as ratio of phosphorylated to total protein. The ratio was normalized to control. Data are mean ± SE; 𝑛=3. *𝑃<0.05 versus control (0 min).

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