Extracellular Signal-Regulated Kinase Is a Direct Target of the Anti-Inflammatory Compound Amentoflavone Derived from Torreya nucifera
Figure 1
Effect of amentoflavone and Tn-EE-EA on the production of inflammatory mediators. (a) Chemical structure of amentoflavone (AF). (b, c, and g) Production of NO and PGE2 in RAW264.7 cells treated with LPS (1 μg/mL) or pam3CSK (10 μg/mL) in the presence or absence of amentoflavone (from 0 to 200 μM) or quercetin (25 μM) was measured using the Griess assay and EIA. ((d) and (e), right panel) The viability of RAW264.7 cell treated with amentoflavone (from 0 to 400 μM) or Tn-EE-EA (0 to 200 μg/mL) in the presence or absence of LPS was determined using an MTT assay. (e) Production of NO in RAW264.7 cells treated with LPS (1 μg/mL) in the presence or absence of Tn-EE-EA (from 0 to 200 μg/mL) was measured using the Griess assay (left panel). (f) The content of amentoflavone from Tn-EE-EA was analysed using a high-performance liquid chromatograph (HPLC) (Knauer). and compared to the control.
