Differential Regulation of Inflammation and Immunity in Mild and Severe Experimental Asthma

Table 3

Relative T-cell transcription factor mRNA expression in mouse whole lung tissue during mild and severe allergic asthma.

Transcription factor	Model	Control; a	Sensitized only; b	Challenged only; c	Sensitized and challenged
Tbet	Mild	1.00 ± 0.40	1.06 ± 0.35	4.56 ± 2.23	2.32 ± 0.51
	Severe	1.00 ± 0.19	1.05 ± 0.31	1.45 ± 0.02	15.94 ± 5.09^a,b,c
Gata3	Mild	1.00 ± 0.01	0.68 ± 0.16	1.10 ± 0.14	1.44 ± 0.45
	Severe	1.00 ± 0.17	0.67 ± 0.08	0.57 ± 0.06	0.84 ± 0.21
Rorγt	Mild	1.00 ± 0.11	0.67 ± 0.33	0.72 ± 0.15	0.35 ± 0.02
	Severe	1.00 ± 0.02	0.71 ± 0.19	1.02 ± 0.14	0.117^d
Foxp3	Mild	1.00 ± 0.31	1.87 ± 0.50	0.95 ± 0.34	8.55 ± 2.82^a
	Severe	1.00 ± 0.11	0.92 ± 0.21	2.03 ± 0.39	17.11 ± 1.06^a,b,c
Ratio
Gata3/Tbet	Mild	1.00 ± 0.10	0.64 ± 0.26	0.24 ± 0.03	0.10 ± 0.03^a,b
	Severe	1.00 ± 0.17	0.63 ± 0.07	0.30 ± 0.03	0.05 ± 0.01^a,b
Foxp3/Rorγt	Mild	1.00 ± 0.31	2.78 ± 0.63	4.33 ± 3.05	37.12 ± 12.24^a,b,c
	Severe	1.00 ± 0.11	1.29 ± 0.29	2.00 ± 0.38	439.08 ± 27.31^a,b,c
Foxp3/Gata3	Mild	1.00 ± 0.31	2.77 ± 0.63	2.83 ± 1.98	5.95 ± 1.96^a
	Severe	1.00 ± 0.11	1.37 ± 0.31	3.55 ± 0.67	20.40 ± 1.27^a,b,c
Foxp3/Tbet	Mild	1.00 ± 0.31	1.77 ± 0.50	0.69 ± 0.48	0.61 ± 0.20
	Severe	1.00 ± 0.11	0.87 ± 0.20	1.07 ± 0.20	1.07 ± 0.07

Tbet, Gata3, Rorγt, and Foxp3 represent Th1, Th2, Th17, and Treg cells, respectively. The values shown in the table are relative to transcription factor mRNA expression levels in control mice. Data is shown as mean ± SEM. Ratios for Th2/Th1 (Gata3/Tbet), Treg/Th17 (Foxp3/Rorγt), Treg/Th2 (Foxp3/Gata3), and Treg/Th1 (Foxp3/Tbet) mRNA expression are also shown. The mean ratio was calculated by dividing the individual expression values for the first transcription factor (numerator) by the mean expression value for the second transcription factor (denominator). Statistical significance of differences was tested using Bonferroni’s post hoc test after one-way ANOVA. ^a𝑃<0.05 compared to control mice; ^b𝑃<0.05 compared to sensitized only mice; ^c𝑃<0.05 compared to challenged only mice; ^da single value.