Research Article

Clearance of Apoptotic Cells by Macrophages Induces Regulatory Phenotype and Involves Stimulation of CD36 and Platelet-Activating Factor Receptor

Figure 4

Efferocytosis-induced IL-10 and IL-12p40 production. Apoptotic thymocytes were added to BMDM (10 per macrophage), and after 24 h the supernatants were assayed (a). Another group, after 24 h of contact with apoptotic cells, was stimulated with LPS (10 ng/mL), and supernatants were assayed after 24. Treatments with PAFR antagonists WEB (WEB2086, 50 μM) or CV (CV3988, 10 μM) and with specific blocking antibody to CD36 (1 μg/mL) were performed 30 min prior to the addition of apoptotic thymocytes. IL-10 and IL-12p40 levels in the cultures supernatants were assessed by ELISA according to manufacturer’s specifications. Data are presented as the mean ± SEM ( versus control without AC; versus control with apoptotic cells).