Posttranslational Nitration of Tyrosine Residues Modulates Glutamate Transmission and Contributes to N-Methyl-D-aspartate-Mediated Thermal Hyperalgesia

Table 1

Densitometry data expressed in %.

	Treatment	Nitrated protein	Total lysate	β-actin
GluN1	Naive	17.00±3.58	31.56±7.8	32.26±7.3
	NMDA (2 nmol, i.t.)	60.00±2.94^†	35.44±5.5	33.53±7.5
	NMDA + FeTMPyP (2 nmol, i.t.)	23.48±1.43^∗	33.00±3.5	34.21±6.2
GluN2B	Naive	14.67±1.96	34.00±3.5	32.26±7.3
	NMDA (2 nmol, i.t.)	65.58±3.35^†	33.73±3.7	33.53±7.5
	NMDA + FeTMPyP (2 nmol, i.t.)	19.75±2.28^∗	32.27±2.5	34.21±6.2
GLT-1	Naive	14.78±2.34	34.22±3.5	33.98±2.3
	NMDA (2 nmol, i.t.)	70.31±2.35^†	34.36±4.5	33.10±5.8
	NMDA + FeTMPyP (2 nmol, i.t.)	14.91±2.18^∗	31.42±4.8	32.92±6.8
GS	Naive	5.14±1.12	33.10±4.2	32.78±4.4
	NMDA (2 nmol, i.t.)	84.46±2.19^†	33.40±3.1	32.67±6.7
	NMDA + FeTMPyP (2 nmol, i.t.)	10.40±0.1^∗	33.50±2.8	34.55±4.3

^†P < 0.001 compared to vehicle and *P < 0.001 compared to NMDA alone.