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Mediators of Inflammation
Volume 2013 (2013), Article ID 953841, 19 pages
http://dx.doi.org/10.1155/2013/953841
Research Article

Ubiquitous Transgenic Overexpression of C-C Chemokine Ligand 2: A Model to Assess the Combined Effect of High Energy Intake and Continuous Low-Grade Inflammation

1Unitat de Recerca Biomèdica, Hospital Universitari Sant Joan, Institut d’Investigació Sanitària Pere Virgili, Universitat Rovira i Virgili, Carrer Sant Llorenç 21, 43201 Reus, Spain
2Campus of International Excellence Southern Catalonia, Spain
3Catalan Institute of Oncology and Girona Biomedical Research Institute, Avda de Francia s/n, 17007 Girona, Spain
4Department of Pathology, Hospital Universitari Joan XXIII, C/ Dr. Mallafrè Guasch 4, 43005 Tarragona, Spain
5Department of Vascular Surgery, Hospital Universitari Joan XXIII, C/ Dr. Mallafrè Guasch 4, 43005 Tarragona, Spain
6Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Ramiro de Maeztu 9, 28040 Madrid, Spain
7Servei de Medicina Interna, Hospital Sant Pau i Santa Tecla, Rambla Vella 14, 43003 Tarragona, Spain

Received 22 July 2013; Revised 30 September 2013; Accepted 15 October 2013

Academic Editor: Donna-Marie McCafferty

Copyright © 2013 Esther Rodríguez-Gallego et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Material S1: comparison between human and murine CCL2 regions, corresponding to the manuscript.

Supplementary Material S2: details of the sequence of other main elements used in the construction of the vector, corresponding to the manuscript.

Supplementary Material S3: details on the construct design, corresponding to the manuscript.

Supplementary Material S4: Weight of selected tissues and organs, corresponding to the manuscript.

Supplementary Material S5: Weight of adipose tissues in the three strains after 14 weeks of a chow diet or high fat diet treatment.

Supplementary Material S6: disturbances in glucose metabolism, corresponding to the manuscript.

Supplementary Material S7: expression of fatty acid synthase and AMPK in the liver, corresponding to the manuscript.

Supplementary Material S8: dietary fat and overexpression of CCL2 modify the size, number and morphology of liver macrophages also after 14 weeks of dietary treatment corresponding to the manuscript.

Supplementary Material S9: Transgenic mice that overexpress CCL2 die prematurely when fed high-fat diet, corresponding to the manuscript.

  1. Supplementary Material