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Mediators of Inflammation
Volume 2014, Article ID 102160, 13 pages
Research Article

5-Lipoxygenase-Dependent Recruitment of Neutrophils and Macrophages by Eotaxin-Stimulated Murine Eosinophils

1Department of Immunology, IMPG, Universidade Federal do Rio de Janeiro, CCS, Bloco I, Room I-2-066, 21941-590 Rio de Janeiro, Brazil
2Department of Pediatrics, IFF, FIOCRUZ, 22250-020 Rio de Janeiro, Brazil
3Department of Medical Microbiology, IMPG, Universidade Federal do Rio de Janeiro, 21941-590 Rio de Janeiro, Brazil

Received 14 November 2013; Revised 14 January 2014; Accepted 15 January 2014; Published 25 February 2014

Academic Editor: Shaoheng He

Copyright © 2014 Ricardo Alves Luz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Figure: Representative image of the mixed leukocyte infiltrates collected from BALB/c mice, 4 h after i. p. injection of 50 ng eotaxin, and stained with Giemsa. In the center, one eosinophil, recognizable by its orange-stained cytoplasm and donut-shaped nucleus, without segmentation. The field contains three neutrophils, recognizable by pale staining of the cytoplasm and by clearly segmented nuclei, ranging from strongly stained, condensed chromatin, to pyknosis. There is one recognizable lymphocyte at the center. The field contains numerous cells with monocyte/macrophage morphology, which are larger than granulocytes, show clearly-stained cytoplasm, oval or round nuclei with loosely condensed chromatin. In the same page, please note that Figure 6(i) has been modified in a way that makes it difficult for the reader to identify which or the two continuous lines shown is thick, which is thin, as they appear very similar. I would suggest that you refer to the files sent you to make sure you, in which the thick line is the higher continuous line, and there is no confusion possible, and try to depict these two continuous lines in a more distinctive way, to avoid confusing the readers.

  1. Supplementary Figure