Figure 8: Effect of DEC on immunohistochemical localization of iNOS in lung tissue after pleurisy induced by carrageenan. (a) In tissue sections from the CAR group, positive labeling was detected on alveolar macrophages. (b) Treatment with DEC significantly reduced the iNOS staining in comparison to the CAR group, achieving levels similar to the sham group. (c) Densitometry analysis of immunohistochemistry photographs for iNOS from lung tissues; figure representative of at least 3 experiments performed on different experimental days; data expressed as mean ± S.E.M. from mice for each group; versus carrageenan; scale bar = 20 μm.