An Asp49 Phospholipase A2 from Snake Venom Induces Cyclooxygenase-2 Expression and Prostaglandin E2 Production via Activation of NF-κB, p38MAPK, and PKC in Macrophages
Figure 2
NF-B is involved in COX-2 expression and PGE2 release induced by MT-III in macrophages. Resident peritoneal macrophages ( cells) were pretreated with TPCK (2.5 M) or SN50 (50 μg/mL) and incubated during 4.5 h with MT-III (0.4 M). (a, c) Western blotting of COX-2 and -actin (loading control) of cells pretreated with SN50 or TPCK. (b, d) Densitometric analysis of immunoreactive COX-2 band intensities. (e) PGE2 was quantified in culture supernatants by enzyme immunoassay (see Section 2). Results are expressed as mean ± SEM from 3 experiments. * as compared with control value.