Anti-Inflammatory Mechanism of Polyunsaturated Fatty Acids in Helicobacter pylori-Infected Gastric Epithelial Cells
Figure 5
Effects of PUFAs on EGFR activation in H. pylori-infected AGS cells. (a) AGS cells were infected with H. pylori for the indicated time points (7.5, 15, 30, 60, and 120 min). Phosphospecific and total forms of EGFR were determined by western blotting. (b) AGS cells were pretreated with various concentrations of tyrphostin AG1478 (0.04, 0.2, and 1 M), a specific EGFR inhibitor, for 1 h prior to H. pylori stimulation The cells were then infected with H. pylori for 30 min and phospho-ERK and phospho-JNK levels were determined by western blotting. (c) AGS cells were pretreated with individual fatty acid (100 M) for 24 h and were then infected with H. pylori for 15 min.
