Myeloperoxidase Oxidized LDL Interferes with Endothelial Cell Motility through miR-22 and Heme Oxygenase 1 Induction: Possible Involvement in Reendothelialization of Vascular Injuries

Figure 3

The effect of Mox-LDL treatment on wound closure after HUVEC monolayer scratch. (a) Scratched monolayers of HUVEC cells. HUVEC were seeded, allowed to reach confluence, and incubated for 24 hours more. HUVECs were then either treated or not with native LDL or Mox-LDL for 24 h. Wounds were then made by scratching the confluent cell monolayers with a yellow pipet tip and washed twice and cells were incubated for additional 8 hours in the same conditions. Pictures were taken at time of wounding and eight hours later in all conditions. Black lines defining wound edges were superimposed to the pictures. The results shown are representative of three independent experiments performed in duplicate. (b) Quantification of (a). After scratching the HUVEC monolayers to induce wounds, cells were incubated for additional 8 hours in the above conditions, pictures were taken, and the distance between the wound edges were measured in all conditions. The distance that remained cell-free at  h was calculated as a percentage of the initial wound width at time of scratching  h. Mean ± SEM on 3 independent experiments performed in duplicate. versus control (ANOVA, Turkey’s multiple comparison test).

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