Mediators of Inflammation / 2014 / Article / Fig 6

Research Article

Effect of Tumor Necrosis Factor Family Member LIGHT (TNFSF14) on the Activation of Basophils and Eosinophils Interacting with Bronchial Epithelial Cells

Figure 6

Phosphorylation of p38 MAPK, ERK1/2, and IκBα in BEAS-2B cells upon the coculture of basophils/eosinophils and BEAS-2B cells with the stimulation of LIGHT. BEAS-2B cells (1 × 105 cells) and basophils/eosinophils (3 × 105 cells) were cultured either together or separately with or without LIGHT stimulation (100 ng/mL) for different time points (0, 15, 30, and 60 min). The intracellular expression of phosphorylated (p) p38 MAPK, pERK1/2, and pIκBα in (a) permeabilized basophils and (b) eosinophils alone without coculture, and (c) pp38 MAPK, (d) pERK1/2, and (e) pIκBα of permeabilized BEAS-2B cells in with or without coculture with basophils/eosinophils were measured by intracellular immunofluorescence staining using flow cytometry. Results are shown in MFI and expressed as the arithmetic mean plus SEM of three independent experiments with three blood samples in bar charts. * , ** when compared with coculture control group. BAS: basophils; Eosi: eosinophils; BS: BEAS-2B cells.

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