Early Activation of Pulmonary TGF-β1/Smad2 Signaling in Mice with Acute Pancreatitis-Associated Acute Lung Injury
Figure 2
Expression level of the TGF-β receptors. Representative images of the levels of TβRII at 9 h (a and b) and ALK5/TβRI at 9 h (c and d) and 24 h (e-f), and in lung tissue from sham controls (a, c, and e) and after acute pancreatitis induction (b, d, and f). Results from one representative of eight samples per group are shown. Original magnification, ×20. Number of ALK5-positive cells in the lungs of sham controls and ligated groups at 9 and 24 h (g). Graph represents number of positive cells as mean ± SEM in 10 microscope fields (×20 magnification), per group. *; *** versus sham control, two-tailed Student’s t-test. Western blot analysis of total ALK5 protein levels in lung extracts of sham-operated and ligated animals at the early 9 h and advanced 24 h time points (h). Two of five similar analyses per group are shown.
