Resolution of Sterile Inflammation: Role for Vitamin C
Figure 3
LPS differentially activates proinflammatory gene expression in macrophages from vitamin C sufficient and deficient mice. Peritoneal macrophages elicited on day 3 following TG-induced peritonitis from VitC sufficient and deficient Gulo−/− mice were exposed to LPS (50 ng/mL) for 4 hours. Macrophages from some VitC deficient mice were incubated with AscA (3 mM, 16 hours) prior to LPS exposure (deficient + AscA). Real time QPCR was performed for IL-1β (a) and TNFα (b) (/group). (c) Upper panel: representative western blot for expression of phospho-NFκB and NFκB from VitC deficient macrophages exposed to media alone (M), AscA (3 mM, 16 hours (AscA)), LPS (50 ng/mL) for 1 hour (LPS), or AscA for 16 hours followed by LPS for 1 hour (LPS + AscA). Lower panel: densitometry for normalized expression of phospho-NFκB from macrophages (/group). (d) Upper panel: representative western blot for expression of iNOS and actin from macrophages groups described in (c) and exposure to LPS (50 ng/mL) for 4 hour. Lower panel: densitometry for normalized expression of iNOS from macrophages (/group).
