Generation of IL-8 and IL-9 Producing CD4+ T Cells Is Affected by Th17 Polarizing Conditions and AHR Ligands
Figure 1
Effect of Th17 polarizing conditions on the T helper cell cytokine production. Human CD4+ T cells were cultured with anti-CD3/anti-CD28 in the presence or absence of Th17 polarizing conditions for five days. After restimulation with PMA, Ionomycin, and Monensin for five hours and fixation/permeabilization the cells were stained for IL-17A, IL-22, IL-9, IL-8, and IFN-γ and analyzed by flow cytometry. (a) The numbers in the dotplots represent positive cells in %. Data are representative of 3–5 independent experiments. (b) The amount of cytokine producing cells [%] is represented as mean and SEM. Bars with values indicate significant differences between the two groups, Mann-Whitney test, .
