The First Trimester Gravid Serum Regulates Procalcitonin Expression in Human Macrophages Skewing Their Phenotype In Vitro
Figure 4
The expression of PCT is negatively regulated in human macrophages conditioned for 24 hours with gravid serum (GS), reaching significance after stimulation with 100 ng/mL of LPS for 3 hours. mRNA expression levels of these proteins were measured by qPCR and normalized to those of the human housekeeping gene 18S. Results were expressed as AUs, in which 1 AU represents the value obtained with macrophages cultured in NHS used as a positive control. Bars represent the mean ± S.E.M. of at least 3 independent experiments. (Mann-Whitney test).
