The First Trimester Gravid Serum Regulates Procalcitonin Expression in Human Macrophages Skewing Their Phenotype In Vitro

Table 1

Primer used for qPCR analysis.

Gene	Primers	Sequence 5′→3′	Annealing temperature (°C)	Amplicon size (bp)	Gene bank accession number
18S	For	ATCCCTGAAAAGTTCCAGCA	60	154	NM_022551
	Rev	CCCTCTTGGTGAGGTCAATG
PCT	For	TCTAAGCGGTGCGGTAATCTG	60	85	NM_001741
	Rev	CAGTTTGGGGGAACGTGTGA
IL-1𝛽	For	TTCCCTGCCCACAGACCTTC	66	298	NM_000576
	Rev	AGGCCCAAGGCCACAGGTAT
IL-10	For	CCAAGCCTTGTCTGAGATGAT	61	120	NM_000572
	Rev	CTGAGGGTCTTCAGGTTCTCC
CD80	For	AGGAACACCCTCCAATCTCTG	60	150	NM_005191
	Rev	GGTCAAAAGTGAAAGCCAACA
CD206	For	TATGGAATAAAGACCCGCTGAC	61	133	NM_002438
	Rev	TGCTCATGTATCTCTGTGATGCT