MicroRNA Profiling of Activated and Tolerogenic Human Dendritic Cells
Figure 4
Comparison of miRNA profiles in activated versus tolerogenic DCs after 6 h and 24 h of maturation. Immature iDCs were cultured with GM-CSF (800 UI/mL) and IL-4 (500 UI/mL) for 6 days. On day 6, the media were replaced for iDCs containing LPS/IFN-γ (aDCs) or containing IL-10/TGF-β (tDCs) for next 6 h or 24 h maturation. The q-PCR based TLDA miRNA expression analysis was provided in all DCs samples. Gene expression values for miRNAs were normalized using RNU6B as an endogenous control. The relative expression levels of target miRNAs were determined by the equation , in which ΔCt were calculated as follows: ΔCt = CtmiR-of-interest − Ct RNU6B. The observed changes in miRNA expression with fold change higher than twofold were analyzed. The results are visualized through heat maps and dendrograms. Green-red colour scale display in the heat maps increasing miRNA in measured samples. (a) miRNA expression in 6 h aDCs versus 6 h tDCs. (b) miRNA expression in 24 h aDCs versus 24 h tDCs. (c) Table summarizing upregulated and downregulated miRNAs in aDCs and tDCs upon 6 and 24 h of maturation and iDCs.
