HO-1 Induction by CO-RM2 Attenuates TNF-α-Induced Cytosolic Phospholipase A2 Expression via Inhibition of PKCα-Dependent NADPH Oxidase/ROS and NF-κB
Figure 6
Regulation of cPLA2 expression in TNF-α-treated mice. (a) Immunohistochemical staining for cPLA2 and vimentin and hematoxylin and eosin (H&E) staining in serial sections of ankle joints from PBS-treated mice (sham; (A)–(C)), TNF-α-injected mice (TNF-α; (D)–(F)), Gö6976-pretreated mice (Gö/TNF-α; (G)–(I)), NAC-pretreated mice (NAC/TNF-α; (J)–(L)), and helenalin-pretreated mice (HLN/TNF-α; (M)–(O)) are shown. Microscopic observation showed vimentin in the synovial membrane of ankle joints, and synovial fibroblasts overlapping with cPLA2 expression. Arrowheads indicate positive staining. (b) The diagrammatic representation of quantitative data of cPLA2-positive cells in the articular joints of mice injected with the indicated inhibitors. Results are representative of 3 mice per experimental group. In (b), values are the mean ± SEM. , as compared with the cells exposed to TNF-α alone.
