Astrocyte Elevated Gene-1 Mediates Glycolysis and Tumorigenesis in Colorectal Carcinoma Cells via AMPK Signaling
Figure 2
AEG-1 promoted anaerobic glycolysis via AMPK in NCM460 human colonic epithelial cells. (a) Western blots showing the overexpression of myc-AEG-1 proteins in NCM460 cells. ((b)-(c)) Biochemical assays showing the increasing of glucose consumptions (b) and lactate productions (c) in AEG-1-overexpressed NCM460 cells, in contrast to controls. Results were averages of four independent experiments. Data represent mean ± SEM. **. (d) Western blots showing that AMPK phosphorylation and PFK2 phosphorylation were increased in AEG-1 overexpressed NCM460 cells. It is noted that the total AMPK or PFK2 protein levels were not altered by AEG-1 overexpression.
