Research Article

Astrocyte Elevated Gene-1 Mediates Glycolysis and Tumorigenesis in Colorectal Carcinoma Cells via AMPK Signaling

Figure 3

Inhibition of AMPK signaling reverses AEG-1-mediated increasing of glycolysis. (a) Western blots showing that inhibition of AMPK activity by Compound C (20 μM, 2 h) reduced AMPK phosphorylation and PFK2 phosphorylation in both AEG-1-overexpressed and nontransfected NCM460 cells. (b) Biochemical assays showing that inhibition of AMPK activity ameliorated AEG-1-induced glucose consumption and lactate production in NCM460 cells. Results were averages of four independent experiments. Data represent mean ± SEM. * , ** .
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