Astrocyte Elevated Gene-1 Mediates Glycolysis and Tumorigenesis in Colorectal Carcinoma Cells via AMPK Signaling
Figure 4
Downregulation of AEG-1 expression reverses AEG-1-mediated increasing of glycolysis in HCT116 tumor cells. (a) Western blots showing that decreasing AEG-1 protein levels by RNAi reduced AMPK phosphorylation and PFK2 phosphorylation in HCT116 tumor cells. (b) Biochemical assays showing that decreasing AEG-1 protein levels by RNAi reduced glucose consumption and lactate production in HCT116 cells. Results were averages of four independent experiments. Data represent mean ± SEM. **. (c) Images showing that the decreased AEG-1 protein levels may inhibit cell migrations in HCT116 cells. (d) Schematic representation highlighting the molecular link between AEG-1 and glycolysis. AEG-1 may increase the activity of AMPK by Thr172 phosphorylation. The activated AMPK then raises PFK2 activity and promotes glycolysis, as is called Warburg effect.
