Induction of Heme Oxygenase-1 with Hemin Reduces Obesity-Induced Adipose Tissue Inflammation via Adipose Macrophage Phenotype Switching

Figure 5

HO-1 induction induces M2 macrophage polarization in adipose tissue. C57BL/6 mice were fed an HFD diet for 2 weeks with hemin alone or hemin in combination with ZnPP injection for 3 times per week (). (a) The level of HO-1 transcripts in adipose tissue was detected by qRT-PCR. (b) Total RNAs were isolated and the levels of M1 marker (CD274, NOS2) and M2 markers (Mrc1, Clec10a) were analyzed by qRT-PCR; β-actin was used as control gene. A.U.: arbitrary units. (c) Levels of HO-1, PPARγ, and pSTAT6/STAT6 were detected by western blotting. Results are means ± SEM. , , and (compared with HFD-vehicle control).

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