The Study of Mechanisms of Protective Effect of Rg1 against Arthritis by Inhibiting Osteoclast Differentiation and Maturation in CIA Mice
Figure 5
Effects of Rg1 on RANKL-induced IκB, NF-κB, and MAP kinase activation. IκB, NF-κB, and MAP kinase activation was represented by the levels of protein phosphorylation. RAW 264.7 cells (1 × 105 cells/mL) were cultured with or without Rg1 (100 μg/mL) in the presence of RANKL for indicated times. Western blot analysis was performed with whole cell lysates (10 mg). Blots were probed with antibodies specific for IκB, NF-κB, and MAP kinase. The densities of phosphorylated protein (p-) levels (upper panels) were normalized to the density of total protein levels (lower panels). GAPDH was used as the loading control.
